6J9T
Complex structure of Lactobacillus casei lactate dehydrogenase with fructose-1,6-bisphosphate
Replaces: 3VKVExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.250, 83.582, 180.094 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 41.180 - 2.700 |
| R-factor | 0.19712 |
| Rwork | 0.196 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zqz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.076 | 0.367 |
| Number of reflections | 67283 | 9734 |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 100mM sodium citrate, 200mM potassium sodium tartrate, 1.6M ammonium sulfate, 2mM fructose-1,6-bisphosphate |
