6J9R

Coiled-coil Domain of Drosophila TRIM Protein Brat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	51.612, 61.100, 128.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.186 - 2.500
R-factor	0.2259
Rwork	0.224
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.089	0.067	0.373
Rmeas	0.093	0.070	0.389
Rpim	0.026	0.020	0.106
Number of reflections	14770	833	712
<I/σ(I)>	7.4
Completeness [%]	100.0	100	100
Redundancy	12.8	11.3	13.3
CC(1/2)		0.998	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	291.15	0.2M NaCl,0.1M Na2(CH3COO).3H2O,30% MPD