6J9C

Crystal structure of Arabidopsis thaliana transcription factor LEC2-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	71.073, 96.601, 99.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.591 - 3.102
R-factor	0.2263
Rwork	0.225
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9a
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.146	0.527
Number of reflections	8746	1271
<I/σ(I)>	9.4	3.56
Completeness [%]	99.5	99.8
Redundancy	5.4	5.7
CC(1/2)		0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M sodium HEPES pH 7.5, 15% PEG 20000