6J8Y

Crystal structure of the human RAD9-HUS1-RAD1-RHINO complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	52.937, 136.190, 154.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.802 - 2.400
R-factor	0.2205
Rwork	0.218
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a1j
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.066	0.779
Number of reflections	44421	27653
<I/σ(I)>	21.17
Completeness [%]	99.6
Redundancy	6.68
CC(1/2)	0.999	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Sodium citrate, Bis-tris propane pH 7.5