6J8F

Crystal structure of SVBP-VASH1 with peptide mimic the C-terminal of alpha-tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	66.950, 108.377, 46.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.188 - 2.283
R-factor	0.2015
Rwork	0.200
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	low resolution SeMet structure
RMSD bond length	0.003
RMSD bond angle	0.685
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.14_3260: ???))
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.190	2.410
High resolution limit [Å]	2.280	2.280
Rpim		0.491
Number of reflections	16147	2322
<I/σ(I)>	10.9	2.2
Completeness [%]	99.9	100
Redundancy	12.5	12.9
CC(1/2)		0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Na citrate tribasic dihydrate pH 5.0, 18% PEG 20000