6J80
Human mitochondrial Oligoribonuclease in complex with poly-dT DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-06 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.079, 88.403, 45.939 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 26.115 - 1.812 |
| R-factor | 0.1941 |
| Rwork | 0.193 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2igi |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
| High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
| Rmerge | 0.027 | 0.012 | 0.286 |
| Number of reflections | 45376 | 2276 | 2149 |
| <I/σ(I)> | 38.3 | ||
| Completeness [%] | 99.4 | 97.1 | 93.7 |
| Redundancy | 3.6 | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2 M Ammonium citrate dibasic, 20% w/v PEG 3350 |
