6J7W

Crystal Structure of Human BCMA in complex with UniAb(TM) VH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95
Spacegroup name	C 2 2 21
Unit cell lengths	65.392, 73.806, 133.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 2.600
R-factor	0.2118
Rwork	0.208
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zhk 4zfo
RMSD bond length	0.009
RMSD bond angle	1.350
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	2.700
High resolution limit [Å]	2.590	2.590
Rmerge	0.077	0.268
Rmeas	0.083	0.289
Rpim	0.032	0.108
Number of reflections	10364	1219
<I/σ(I)>	11.6
Completeness [%]	99.8	99.4
Redundancy	6.6	7
CC(1/2)	0.998	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	0.2M sodium malonate pH 4.0 and 20% PEG 3350