6J7L

Crystal structure of Pseudomonas aeruginosa Earp in complex with TDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.988
Spacegroup name	P 21 21 21
Unit cell lengths	45.322, 92.002, 119.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.478 - 1.851
R-factor	0.1708
Rwork	0.169
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j7j
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.075	0.042	0.690
Rmeas	0.078	0.044	0.717
Rpim	0.021	0.012	0.192
Total number of observations	567700
Number of reflections	43403	4620	4266
<I/σ(I)>	4.1
Completeness [%]	100.0	100	100
Redundancy	13.1	12.4	13.5
CC(1/2)		0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH6.5, 12% PEG20K