6J7K

Crystal structure of Pseudomonas aeruginosa Earp in complex with TDP-Rha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	45.521, 92.366, 119.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.638 - 2.150
R-factor	0.2188
Rwork	0.217
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j7j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.090	0.042	0.569
Rmeas	0.098	0.045	0.615
Rpim	0.038	0.017	0.228
Total number of observations	186832
Number of reflections	28155	1570	1397
<I/σ(I)>	4.9
Completeness [%]	98.7	99.8	98.9
Redundancy	6.6	6.5	6.9
CC(1/2)		0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES pH6.5, 12% PEG20K