6J7G

Human H-ferritin mutant-C90A/C102A/C130A/D144C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-05-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9979
Spacegroup name	P 1 21 1
Unit cell lengths	117.380, 165.364, 164.865
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	40.870 - 3.868
R-factor	0.2477
Rwork	0.243
R-free	0.32790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
RMSD bond length	0.014
RMSD bond angle	1.532
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.870	4.900
High resolution limit [Å]	3.860	3.860
Number of reflections	54320
<I/σ(I)>	2.7
Completeness [%]	92.0
Redundancy	2.3
CC(1/2)	0.921	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	Amonium Sulfate, HEPES