6J7E
Crystal Structure of Central domain of FleQ in complex with ATPgS and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97319 |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.549, 104.549, 42.762 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.274 - 2.400 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5exp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.600 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.663 |
| Number of reflections | 10591 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 0.2M of sodium thiocyanate, 22% of PEG 3350 |
