6J79

Fusion protein of heme oxygenase-1 and NADPH-cytochrome P450 reductase (13aa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-26
Detector	RAYONIX MX225HE
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	83.360, 159.780, 189.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.674 - 3.331
R-factor	0.2042
Rwork	0.202
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wkt
RMSD bond length	0.002
RMSD bond angle	0.641
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.330	3.330
Number of reflections	37461	2499
<I/σ(I)>	14.04
Completeness [%]	99.0	91.4
Redundancy	8.06	7.57
CC(1/2)	0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 20000, MES-NaOH, ethyl acetate