6J75

Structure of 3,6-anhydro-L-galactose Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-12-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	103.390, 132.355, 159.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.243 - 2.695
R-factor	0.1604
Rwork	0.158
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k2w
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.792
High resolution limit [Å]	2.695	2.695
Rmerge	0.174	0.431
Number of reflections	60262	5280
<I/σ(I)>	27.46
Completeness [%]	99.0
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	NaAC, sodium cacodylate trihydrate, PEG 8,000