6J6V

Crystal structure of heat shock factor 4-DBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-10-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	I 2 2 2
Unit cell lengths	44.619, 68.229, 80.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.979 - 1.200
R-factor	0.1337
Rwork	0.133
R-free	0.15140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.980	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	36700
<I/σ(I)>	49.3
Completeness [%]	99.3
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		295	sodium nitrate