6J6P

Crystal structure of diamondback moth ryanodine receptor phosphorylation domain(2836-3050) mutant S2946D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	55.828, 55.828, 131.552
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.481 - 1.530
R-factor	0.1925
Rwork	0.191
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4etv 5c30
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.560
High resolution limit [Å]	1.530	4.150	1.530
Rmerge	0.109	0.049	0.507
Rmeas	0.116	0.052	0.571
Rpim	0.038	0.017	0.257
Number of reflections	36640	2038	1734
<I/σ(I)>	4.7
Completeness [%]	99.6	99.7	94.1
Redundancy	8.3	9	4.6
CC(1/2)		0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.15	2% v/v PEG 400, 0.1M Sodium HEPES pH 7.5, 2.0M Ammonium sulfate