6J68
Structure of KIBRA and LATS1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.314, 62.837, 156.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.035 - 2.495 |
| R-factor | 0.2246 |
| Rwork | 0.222 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.261 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | RESOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.495 | 2.495 |
| Rmerge | 0.102 | 0.982 |
| Number of reflections | 15953 | 713 |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 0.1M Tris, 28% PEG 4000 |
