6J66

Chondroitin sulfate/dermatan sulfate endolytic 4-O-sulfatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	116.426, 107.732, 87.569
Unit cell angles	90.00, 104.04, 90.00

Refinement procedure

Resolution	37.915 - 1.953
R-factor	0.1823
Rwork	0.180
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qzu
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	75093
<I/σ(I)>	26.21
Completeness [%]	99.2	99.6
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100mM HEPS (pH 7.5), 20% (w/v) PEG 10000