6J58

Crystal structure of human HINT1 complexing with AP4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	78.721, 46.422, 64.251
Unit cell angles	90.00, 94.90, 90.00

Refinement procedure

Resolution	24.465 - 1.521
R-factor	0.1574
Rwork	0.157
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eqe
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.042	0.277
Number of reflections	35587	1790
<I/σ(I)>	41.2
Completeness [%]	99.9	100
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	HEPES pH 7.5, PEG 3350