6J57

Crystal structure of fumarylpyruvate hydrolase from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	66.766, 72.173, 119.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.030 - 2.010
R-factor	0.1713
Rwork	0.169
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rr6
RMSD bond length	0.010
RMSD bond angle	1.595
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.720	2.030
High resolution limit [Å]	2.000	2.000
Rpim	0.057	0.203
Number of reflections	38656	1864
<I/σ(I)>	19.04
Completeness [%]	98.5
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ammonium sulfate, HEPES