6J56

Crystal structure of Myosin VI CBD in complex with Tom1 MBM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97774
Spacegroup name	P 1 21 1
Unit cell lengths	40.733, 74.875, 50.873
Unit cell angles	90.00, 106.34, 90.00

Refinement procedure

Resolution	37.438 - 1.798
R-factor	0.1794
Rwork	0.177
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h8d
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.798	1.800
Rmerge	0.056
Rpim		0.149
Number of reflections	26915	1342
<I/σ(I)>	28.14
Completeness [%]	98.5	98.5
Redundancy	6.6	6.2
CC(1/2)		0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 4000, sodium acetate trihydrate, TRIS hydrochloride