6J4R

Structural basis for the target DNA recognition and binding by the MYB domain of phosphate starvation response regulator 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 42 21 2
Unit cell lengths	88.966, 88.966, 143.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.720 - 2.800
R-factor	0.2171
Rwork	0.215
R-free	0.24910
RMSD bond length	0.012
RMSD bond angle	1.629
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.610	75.610	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.111	2.800	4.072
Rmeas	0.116	0.030	4.235
Rpim	0.033	0.010	1.157
Number of reflections	14723	562	2082
<I/σ(I)>	16
Completeness [%]	99.2	99.8	99.2
Redundancy	12.6	9.7	13.2
CC(1/2)	0.999	0.999	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	28%PEG3350 , 0.1M citrate-Na pH 5.5