6J4K

Structural basis for the target DNA recognition and binding by the MYB domain of phosphate starvation response 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	41.344, 140.221, 65.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 1.580
R-factor	0.1926
Rwork	0.191
R-free	0.21730
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.940	47.900	1.610
High resolution limit [Å]	1.580	8.650	1.580
Rmerge	0.043	0.029	1.806
Rmeas	0.045	0.031	2.015
Rpim	0.013	0.009	0.871
Number of reflections	26266	203	1116
<I/σ(I)>	24.1
Completeness [%]	98.7	99.5	88
Redundancy	11.3	9.8	5
CC(1/2)	1.000	0.999	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	2.4M sodium malonate pH 7.0