6J4F

Crystal structure of the AtWRKY2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	38.100, 48.300, 69.100
Unit cell angles	93.70, 87.30, 97.50

Refinement procedure

Resolution	29.580 - 2.400
R-factor	0.1919
Rwork	0.188
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ayd
RMSD bond length	0.009
RMSD bond angle	0.987
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.470
Rmeas	0.084	0.550
Rpim	0.044	0.290
Number of reflections	17894	1813
<I/σ(I)>	9.59	2.13
Completeness [%]	93.5	96.37
Redundancy	3.4	3.6
CC(1/2)	0.995	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Lithium sulfate, 0.1M MES pH 6.0, 20% PEG 4000