6J4F
Crystal structure of the AtWRKY2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.100, 48.300, 69.100 |
| Unit cell angles | 93.70, 87.30, 97.50 |
Refinement procedure
| Resolution | 29.580 - 2.400 |
| R-factor | 0.1919 |
| Rwork | 0.188 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.580 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.470 |
| Rmeas | 0.084 | 0.550 |
| Rpim | 0.044 | 0.290 |
| Number of reflections | 17894 | 1813 |
| <I/σ(I)> | 9.59 | 2.13 |
| Completeness [%] | 93.5 | 96.37 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.995 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Lithium sulfate, 0.1M MES pH 6.0, 20% PEG 4000 |
