6J3G
Crystal structure of an apo form of the glutathione S-transferase, CsGST83044, of Ceriporiopsis subvermispora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.810, 48.645, 74.308 |
| Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
| Resolution | 39.908 - 1.950 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.20240 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.884 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 45954 | |
| <I/σ(I)> | 35.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 8000, 0.1M HEPES-HCl (pH 7.5), 0.2M ammonium sulfate, 20% (v/v) 2-propanol |
