6J3F

Crystal structure of the glutathione S-transferase, CsGST63524, of Ceriporiopsis subvermispora in complex with glutathione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-14
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	73.093, 105.714, 94.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.148 - 2.500
R-factor	0.193
Rwork	0.190
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f03
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	26161
<I/σ(I)>	43.42
Completeness [%]	100.0
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% (w/v) PEG 8000, 0.1M CHAPS/NaOH (pH10.5), 0.2M NaCl