6J3A

Structure of LmbA2991HD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	96.440, 113.177, 116.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.380
R-factor	0.22576
Rwork	0.224
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.381
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.190	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	51650
<I/σ(I)>	8.33
Completeness [%]	99.4
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Tris pH8.0, 28% w/v Polyethylene glycol 4000