6J2Z

AtFKBP53 N-terminal Nucleoplasmin Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	56.590, 97.390, 96.430
Unit cell angles	90.00, 94.12, 90.00

Refinement procedure

Resolution	68.530 - 2.400
R-factor	0.20538
Rwork	0.204
R-free	0.23325
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k5j
RMSD bond length	0.009
RMSD bond angle	1.527
Data reduction software	iMOSFLM (7.1.1)
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0241)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.530	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.509
Rmeas	0.073	0.609
Rpim	0.029	0.240
Number of reflections	40837	27924
<I/σ(I)>	16.5	3.2
Completeness [%]	100.0	100
Redundancy	6.6	6.5
CC(1/2)	0.999	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.2 M DL-Malic acid (pH 7.0), 25% (w/v) PEG 3350