6J27
Crystal structure of the branched-chain polyamine synthase from Thermus thermophilus (Tth-BpsA) in complex with N4-aminopropylspermidine and 5'-methylthioadenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.570, 158.606, 71.936 |
| Unit cell angles | 90.00, 118.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.660 |
| R-factor | 0.16918 |
| Rwork | 0.167 |
| R-free | 0.20618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3m |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.920 |
| Data reduction software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 156847 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Reservoir: 0.04 M sodium chloride , 0.04 M Tris pH 8.0, 27%(v/v) PEG 350MME |
