6J23
Crystal structure of arabidopsis ADAL complexed with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.381, 82.520, 86.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.352 - 1.900 |
| R-factor | 0.1728 |
| Rwork | 0.171 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iv5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.850 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14rc2_3191) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.870 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.138 | 0.138 |
| Number of reflections | 29882 | 2900 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG 800, 0.1 M MES pH 6.0, 0.2 M Calcium acetate |
