6J22
Crystal structure of Bi-functional enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.711, 45.195, 65.800 |
| Unit cell angles | 90.00, 112.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.19891 |
| Rwork | 0.196 |
| R-free | 0.25764 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.721 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.256 |
| High resolution limit [Å] | 2.200 | 2.199 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 93.6 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M sodium acetate, pH 4.6, 1.8 M ammonium sulfate, 1% 1,2-butanediol |
