6J0X

Crystal Structure of Yeast Rtt107 and Mms22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97855
Spacegroup name	P 1 21 1
Unit cell lengths	72.284, 99.669, 167.057
Unit cell angles	90.00, 94.92, 90.00

Refinement procedure

Resolution	49.433 - 2.310
R-factor	0.2053
Rwork	0.204
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j0v
Data scaling software	Aimless (0.5.21)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	166.440	166.440	2.350
High resolution limit [Å]	2.310	12.650	2.310
Rmerge	0.085	0.020	0.982
Rmeas	0.099	0.023	1.312
Rpim	0.049	0.011	0.860
Total number of observations	403753	2638	11112
Number of reflections	102743	676	4956
<I/σ(I)>	14.2	48.1	1
Completeness [%]	99.1	99.3	97.1
Redundancy	3.9	3.9	2.2
CC(1/2)	0.998	0.999	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	25% PEG 3350, 0.1M Bis-Tris pH 6.0, 0.2M magnesium chloride