6J0V
Crystal Structure of Yeast Rtt107
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97855 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.404, 98.905, 87.464 |
Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
Resolution | 49.452 - 2.314 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.26590 |
Structure solution method | SAD |
Data scaling software | Aimless (0.5.21) |
Phasing software | SHELXD |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 98.900 | 98.900 | 2.390 |
High resolution limit [Å] | 2.310 | 9.250 | 2.310 |
Rmerge | 0.070 | 0.032 | 1.130 |
Rmeas | 0.082 | 0.038 | 1.368 |
Rpim | 0.042 | 0.020 | 0.758 |
Total number of observations | 182440 | 2455 | 13131 |
Number of reflections | 49735 | 738 | 4236 |
<I/σ(I)> | 13.3 | 38.3 | 1 |
Completeness [%] | 97.7 | 88.1 | 91 |
Redundancy | 3.7 | 3.3 | 3.1 |
CC(1/2) | 0.998 | 0.997 | 0.391 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.8 | 277 | 1.8M ammonium sulfate, 50mM sodium cacodylate pH 5.8, 5 mM magnesium acetate, 0.5mM spermine |