6J0K
Crystal structure of intracellular B30.2 domain of BTN3A3 mutant in complex with HMBPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-29 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.797, 41.392, 62.490 |
| Unit cell angles | 73.23, 89.87, 68.95 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.25662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n7u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | |
| Number of reflections | 20422 | 2264 |
| <I/σ(I)> | 16.06 | |
| Completeness [%] | 84.2 | |
| Redundancy | 3 | |
| CC(1/2) | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Isopropanol ,Imidazole, PEG 8000 |
