6J0E
Structures of two ArsR As(III)-responsive repressors: implications for the mechanism of derepression
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-03-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.04478, 1.00495 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.770, 46.387, 121.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.790 - 1.600 |
| R-factor | 0.18174 |
| Rwork | 0.178 |
| R-free | 0.26434 |
| Structure solution method | MAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.038 | 0.448 |
| Rmeas | 0.040 | 0.478 |
| Rpim | 0.014 | 0.162 |
| Number of reflections | 29643 | 1315 |
| <I/σ(I)> | 15.9 | 5.3 |
| Completeness [%] | 87.0 | 78.8 |
| Redundancy | 8 | 7.8 |
| CC(1/2) | 0.994 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 294 | 200mM ammonium tartrate, 20% PEG 3350 |
