6J05
Structures of two ArsR As(III)-responsive repressors: implications for the mechanism of derepression
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.922, 42.727, 49.132 |
| Unit cell angles | 90.00, 117.62, 90.00 |
Refinement procedure
| Resolution | 43.530 - 1.860 |
| R-factor | 0.16802 |
| Rwork | 0.165 |
| R-free | 0.23083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1t |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.416 |
| Data reduction software | AUTOMAR |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.530 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.073 | 0.390 |
| Rmeas | 0.080 | 0.449 |
| Rpim | 0.041 | 0.220 |
| Number of reflections | 13774 | 1235 |
| <I/σ(I)> | 7.2 | 1.4 |
| Completeness [%] | 94.4 | 86.1 |
| Redundancy | 4.04 | 3.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 294 | 0.1% Tryptone, 20% PEG 3350 |
