6J04

Crystal structure of full length human LC3B delta G120 mutant (2_125)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97892
Spacegroup name	P 1
Unit cell lengths	41.644, 56.475, 67.040
Unit cell angles	99.27, 97.45, 109.94

Refinement procedure

Resolution	27.621 - 1.899
R-factor	0.212
Rwork	0.209
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ugm
RMSD bond length	0.007
RMSD bond angle	0.784
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.899	4.090	1.900
Rmerge	0.093	0.062	0.562
Rmeas	0.108	0.073	0.657
Rpim	0.055	0.037	0.339
Number of reflections	40763	4045	4131
<I/σ(I)>	6.8
Completeness [%]	93.5	93	94.4
Redundancy	3.6	3.8	3.6
CC(1/2)		0.984	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.16M ammonium sulfate, 0.08M sodium acetate pH4.6, 20% (w/v) PEG4000; 20% (v/v) glycerol