6IZW

Myxococcus xanthus MglA bound to GTP-gamma-S and MglB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979150
Spacegroup name	P 31 2 1
Unit cell lengths	88.482, 88.482, 131.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.241 - 2.400
R-factor	0.2024
Rwork	0.200
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t12
RMSD bond length	0.008
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.241	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.730
Rmeas	0.103	0.799
Rpim	0.044	0.358
Number of reflections	23862	2456
<I/σ(I)>	16.5	3.5
Completeness [%]	100.0	100
Redundancy	9.8	9.9
CC(1/2)	0.998	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	8% PEG 4000, 200mM Ammonium sulphate