6IZF

Structural basis for activity of TRIC counter-ion channels in calcium release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.7712
Spacegroup name	P 63 2 2
Unit cell lengths	127.381, 127.381, 102.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.992 - 2.000
R-factor	0.1848
Rwork	0.184
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyu
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.540	48.540	2.050
High resolution limit [Å]	1.990	8.690	1.990
Rmerge	0.235	0.088	2.218
Rmeas	0.236	0.088	2.226
Rpim	0.015	0.006	0.191
Total number of observations	7908350
Number of reflections	33712	500	2448
<I/σ(I)>	32.1
Completeness [%]	99.6	99.5	95.4
Redundancy	234.6	239.3	121.5
CC(1/2)	1.000	1.000	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG MME 2000