6IZ9
Crystal structure of the apo form of a beta-transaminase from Mesorhizobium sp. strain LUK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 31 |
| Unit cell lengths | 62.397, 62.397, 171.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.220 - 2.199 |
| R-factor | 0.2466 |
| Rwork | 0.241 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.199 | 2.200 |
| Rmerge | 0.144 | 0.517 |
| Number of reflections | 37878 | 1906 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.3 M sodium citrate tribasic dihydrate, 0.1 M HEPES (pH 7.6), 0.1 M cadmium chloride hydrate |
