6IYZ

Structural basis for activity of TRIC counter-ion channels in calcium release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-14
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9785
Spacegroup name	F 41 3 2
Unit cell lengths	268.210, 268.210, 268.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.413 - 2.200
R-factor	0.2522
Rwork	0.251
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyu
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.410	47.410	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.392	0.065	18.658
Rmeas	0.394	0.066	18.721
Rpim	0.032	0.006	1.516
Total number of observations	6242648	80149	544171
Number of reflections	42420	733	3608
<I/σ(I)>	24.3	107	1.1
Completeness [%]	100.0	99.4	99.8
Redundancy	147.2	109.3	150.8
CC(1/2)	0.999	0.999	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	NaH2PO4, K2HPO4, phosphate-citrate pH 4.2