6IYX

Crystal Structure Analysis of a Eukaryotic Membrane Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9798
Spacegroup name	P 63 2 2
Unit cell lengths	126.723, 126.723, 102.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.265 - 1.800
R-factor	0.2026
Rwork	0.202
R-free	0.21230
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	SHELXD
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.270	46.270	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.070	0.028	4.632
Rmeas	0.071	0.028	4.738
Rpim	0.012	0.005	0.955
Total number of observations	1462867
Number of reflections	43142	445	1819
<I/σ(I)>	30.5
Completeness [%]	95.6	99.1	70.2
Redundancy	33.9	28	22.5
CC(1/2)	1.000	0.999	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEGMME2K