6IYU

Crystal Structure Analysis of an Eukaryotic Membrane Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-16
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9798
Spacegroup name	P 63 2 2
Unit cell lengths	127.449, 127.449, 102.141
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.717 - 2.199
R-factor	0.1993
Rwork	0.198
R-free	0.21720
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	SHELXD
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.550	48.550	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.110	0.044	2.463
Rmeas	0.111	0.045	2.497
Rpim	0.018	0.008	0.405
Total number of observations	954527	12636	80991
Number of reflections	25429	444	2159
<I/σ(I)>	28.6	102	2.2
Completeness [%]	100.0	99.3	99.9
Redundancy	37.5	28.5	37.5
CC(1/2)	1.000	1.000	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG 2000 MME