6IYM

Fumarylacetoacetate hydrolase (EaFAH) from psychrophilic Exiguobacterium antarcticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2016-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	120.437, 72.656, 63.374
Unit cell angles	90.00, 101.68, 90.00

Refinement procedure

Resolution	47.910 - 2.100
R-factor	0.20575
Rwork	0.202
R-free	0.26886
RMSD bond length	0.008
RMSD bond angle	1.573
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	30928
<I/σ(I)>	30.2
Completeness [%]	99.1
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M magnesium chloride, 0.1M Tris-HCl pH 8.5, 20% (w/v) PEG 8000