6IY1
Structure of human Ras-related protein Rab11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.606, 150.762, 60.125 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.110 |
| R-factor | 0.22148 |
| Rwork | 0.219 |
| R-free | 0.26108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oiv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.063 | 0.248 |
| Number of reflections | 48648 | 2190 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 91.2 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.945 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15M Li2SO4, 0.1M Glycine, 0.8M NaH2PO4 1.2M Na2HPO4 |
