6IY0

Crystal structure of conserved hypothetical protein SAV0927 from Staphylococcus aureus subsp. aureus Mu50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	128.181, 76.754, 43.571
Unit cell angles	90.00, 90.35, 90.00

Refinement procedure

Resolution	28.799 - 2.500
R-factor	0.2395
Rwork	0.237
R-free	0.27810
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.518
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.081	0.397
Number of reflections	29554	1495
<I/σ(I)>	18.9	6.7
Completeness [%]	99.3	99.3
Redundancy	6.6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	Tris-HCl pH 7.5, ammonium sulfate