6IXY
X-ray structure of major pilin from C. perfringens SM101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.140, 111.830, 118.590 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 48.710 - 2.720 |
| R-factor | 0.22977 |
| Rwork | 0.227 |
| R-free | 0.27554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xcc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 2.790 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.085 | 0.495 |
| Number of reflections | 69104 | 5092 |
| <I/σ(I)> | 13.8 | 2.9 |
| Completeness [%] | 99.1 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 4% v/v Tacsimate buffer pH 4.0 (73mM malonic acid, 10mM ammonium citrate tribasic, 5mM succinic acid, 12mM D,L-malic acid, 16mM sodium acetate trihydrate, 20mM sodium formate, 6.4mM ammonium tartrate dibasic), 12% w/v PEG 3350 |
