6IXQ

Structure of Myo2-GTD in complex with Smy1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	93.510, 93.510, 204.136
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.717 - 3.060
R-factor	0.2041
Rwork	0.203
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6h
RMSD bond length	0.004
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.280	75.280	3.220
High resolution limit [Å]	3.060	9.660	3.060
Rmerge	0.069	0.043	1.693
Rmeas	0.071	0.045	1.736
Rpim	0.017	0.011	0.378
Number of reflections	20270	738	2911
<I/σ(I)>	20.9
Completeness [%]	99.7	99.5	100
Redundancy	18.7	15.7	20.7
CC(1/2)	0.998	0.997	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	8% w/v PEG 8000, 0.1M sodium dihydrogen phosphate pH 6.5