6IXN
Crystal structure of isocitrate dehydrogenase from Ostreococcus tauri in complex with NAD+ and citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.115, 102.928, 103.290 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.870 |
| R-factor | 0.19663 |
| Rwork | 0.195 |
| R-free | 0.22222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.477 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 171482 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 21% PEG3350, 0.2M (NH4)2SO4, and 0.1M sodium citrate (pH 5.0) |
