6IXL
Crystal structure of isocitrate dehydrogenase from Ostreococcus tauri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.517, 79.273, 112.014 |
| Unit cell angles | 90.26, 102.32, 112.61 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.18491 |
| Rwork | 0.184 |
| R-free | 0.20977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 199682 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 96.4 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 22% PEG8000, 0.1 M (NH4)2SO4 and 0.1 M MES (pH 6.5) |
