6IXF
Crystal structure of SeMet apo SH3BP5 (P41)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 78.212, 78.212, 92.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 3.600 |
| R-factor | 0.2348 |
| Rwork | 0.234 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ixe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.181 | 0.704 |
| Number of reflections | 6560 | 286 |
| <I/σ(I)> | 14.9 | 1.04 |
| Completeness [%] | 97.5 | 92.3 |
| Redundancy | 8.2 | 3.9 |
| CC(1/2) | 0.987 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M succinic acid (pH 7.0), 12% PEG 3350 |
